White kidney bean extract specification



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This general chapter presents 2 sets of tests. The 1 st set gives the reference methods for determining compliance with monographs. Reference to this chapter in a monograph therefore implies compliance with the 1 st set of tests, unless use of the 2 nd set of tests has been authorised. The tests in the 2 nd set also constitute official methods of the European Pharmacopoeia and may be referred White kidney bean extract specification as such, notably in applications for marketing authorisation.

It is intended to replace the 1 st set by the 2 nd set once the monographs concerned have been revised. The 2 nd set presents tests developed in co-operation with the Japanese Pharmacopoeia and the United States Pharmacopeia to achieve harmonised requirements. In this general method the use of certain selective media is proposed. A feature common to all selective media is that sub-lethally injured organisms are not detected.

As sub-lethally injured organisms are relevant for the quality of the product, a resuscitation must be included in examination procedures that rely on selective media. Although the test has been designed to detect bacteria belonging to the family of Enterobacteriaceae, it is recognised that other types of organisms e. Aeromonas, Pseudomonas may be recovered. Detection of bacteria Prepare the product to be examined as described in the general method 2. Subculture on plates of agar medium F.

The product passes the test if there is no growth of colonies of gram-negative bacteria on any plate. Subculture each of the cultures on a plate of agar medium F White kidney bean extract specification obtain selective isolation. Growth of well-developed colonies, generally red or reddish, of gram-negative bacteria constitutes a positive result. Note the smallest quantity of the product that gives a positive result and the largest quantity that gives a negative result. Determine from Table 2.

After incubation, spread on agar medium F for the detection of Enterobacteria and other gram-negative micro-organisms. Prepare the product to be examined as described in the general method 2. Growth of red, non-mucoid colonies of gram-negative rods indicates the possible presence of E. This is confirmed by suitable biochemical tests, such as indole production. The product passes the test if such colonies are not seen or if the confirmatory biochemical tests are negative.

Subculture on at least 2 different agar media chosen from among agar medium J, agar medium K and agar medium L. The probable White kidney bean extract specification of salmonellae is indicated by the growth of cultures having the following appearance: Transfer separately a few of the suspect colonies to agar medium M in tubes, using surface and deep inoculation. The presence of salmonellae is provisionally confirmed if in the deep inoculation but not in the surface culture there is a change of colour from red to yellow and usually a formation of gas, with or without production of hydrogen sulphide in the agar.

Precise confirmation may be carried out by appropriate biochemical and serological tests. The product passes the test if colonies of the type described do not appear or if the confirmatory biochemical and serological tests are negative. If no growth of micro-organisms is detected, the product passes the test. After incubation, spread on agar medium N.

Black colonies of gram-positive cocci surrounded by a clear zone indicate the presence White kidney bean extract specification S. Confirmation may be effected by suitable biochemical tests such as the coagulase test and the deoxyribonuclease test. The product passes the test if colonies of the type described do not appear on agar medium O or if the confirmatory biochemical tests are negative.

After incubation, spread on agar medium O. Dilute portions of each of the cultures using buffered sodium chloride-peptone solution White kidney bean extract specification 7. Mix equal volumes of each suspension and use 0. A positive result for the respective micro-organisms must be obtained.


White kidney bean extract specification








d- Extract treatment: The clarified extract can be treated so as to remove certain impurities, thus improving the blandness, colour and nutritional quality and. Herbal Extracts - Get best quality Herbal Extracts like Amla Extracts, Gymnemma Extract, Senna Extract From Best Moringa Oleifera Manufacturer And Supplier. Copyrights(C) reserved ecoslife.ru: support:ecoslife.ru.

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